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Image Search Results
Journal: Optics Express
Article Title: Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy
doi: 10.1364/OE.541033
Figure Lengend Snippet: MFAO on imaging of Thy1-GFP line M brain slices. (a),(e) Applied artificial astigmatism, pupil-plane corrective phase, and focal-plane SLM1 phase pattern for 0.4-NA Bessel focus by MFAO. (b),(f) Bessel-focus 2PFM images of the neuronal structures without AO, with AO correction in (a),(e), respectively, and without artificial aberration. Lateral pixel size: 0.5 µm. (c),(g) Zoomed-in images of white dashed boxes in (b),(f) (left) and signal profiles along white dashed lines (right). Lateral pixel size: 0.2 µm. (d),(h) Spectral power in the spatial frequency domain of the images in (c),(g) (left) and their radially averaged profiles (right). Dashed circle: (0.667 µm) -1 . Post-objective power: 47.5 mW for (b),(c), 46.5 mW for (f),(g). AU: arbitrary unit.
Article Snippet: Male and
Techniques: Imaging
Journal: Optics Express
Article Title: Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy
doi: 10.1364/OE.541033
Figure Lengend Snippet: MFAO application on in vivo imaging of Thy1-GFP line M mice. (a) Pupil-plane corrective phase and focal-plane SLM1 phase pattern for 0.6-NA Bessel focus by MFAO. (b),(e) Bessel-focus 2PFM images of dendritic structures acquired without and with AO correction in (a). Lateral pixel size: 0.5 µm. (c),(f) Spectral power in the spatial frequency domain of the images in (b),(e) (left) and their radially averaged profiles (right). Dashed circle: (1 µm) -1 . (d),(g) Signal profiles along white dashed lines in (b),(e). (h) Applied artificial astigmatism, pupil-plane corrective phase, and focal-plane SLM1 phase pattern for 0.6-NA Bessel focus by MFAO. (i) Bessel-focus 2PFM images of dendritic structures acquired without and with AO correction in (h) for 0.6-NA Bessel focus. Lateral pixel size: 0.5 µm. (j) Spectral power in the spatial frequency domain of images in (i) (left) and their radially averaged profiles (right). Dashed circle: (1 µm) -1 . (k) Signal profiles along white dashed lines in (i). Post-objective power: 48 mW for (b), 47.8 mW for (e), 47.5 mW for (i). AU: Arbitrary unit.
Article Snippet: Male and
Techniques: In Vivo Imaging